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【共享】经验:The RNeasy Mini Kit from Qiagen

The RNeasy Mini Kit from Qiagen is designed for RNA extraction, although the basic format is similar to other Qiagen products designed to purify nucleic acids: ion exchange columns specifically bind RNA which is purified by wash steps

Plant Seed Direct PCR Kit

实验步骤   1. Protocol for grinding plant seeds.    1) grind using a bead mill       a. place one seed into each well of a 2 ml square well block.       b. Pipette nuclease-free water

Isolation of Total RNA from Animal Cells use RNeasy Mini Kit

using pipet. Do not centrifuge.   5.   Take up to 700µl of that solution and run through a supplied mini column for 15 seconds at 12,000 rpm. Repeat the previous step until all of the solution has run through the mini column.