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文献和实验Fluorometric Quantitation of RNA or DNA
However it also does not give a crude measurement of purity (like an A260 /A280 ratio) nor does it assure that the DNA or RNA is not degraded (e.g. like size determination by gel electrophoesis). Do not use glass (spectrophotmetry) cuvettes in a fluorometer because the frosted glass
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
precipitate the DNA and wash twice with 70% ethanol. Resuspend the DNA at 0.4 -1 ug/ml. Preparing the electroporator There are two types of cuvettes 1 and 2mm. Most Agro protocols use 2mm (Invitrogen #650009 w/blue lids). 1. Make sure power supply
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