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文献和实验in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies
focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.
Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions
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