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- 文献和实验
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上海玉博生物科技有限公司
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文献和实验Protocol for anti-HA antibody Western Blotting
film for ~2 mins. Notes on the primary antibody: Source : Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in -20℃ Internal notes: labelled anti
Antibody Staining of Dissected Gonads
at least 4 hr at RT or overnight at 4 oC (Overnight at 4 oC is probably best). *Wash in PBTw, 3-4 x 5 min *Wash in PBS containing 100 ng/ml DAPI. *Wash in PBTw, once *Place in 80% glycerol containing anti-fade reagent (e.g., Dapco) about 20 30 ul
Protocol for anti-HA antibody Western Blotting
room. Expose film for ~2 mins. Notes on the primary antibody: Source - Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in ―20c
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