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文献和实验Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
only. Procedure Title | Overview | Procedure | Solutions | BioChemicals | Hints | Printable Version A. Filter Preparation (see Hint #2 ) 1. This procedure uses 25 x 80 mm polycarbonate filters, polyvinylpropylene
Differential cDNA Screening Procedures
0.5 ul Incubate at 10-150C overnight continue at RT the next day. 9. Packaging follows next day. * Prepping the Lambda vector before cloning. 1. We prepare 25 ug of lambda vector. The Lambda DNA is (25ug/100 ul of O.1 X TE) heated to 68 C
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
2500rpm 15‚ 4C 8. Repeat 5 and resuspend pellet in 1ml RF2/tube (1/25 vol.) and ice cells 15‚ 9. Aliquot 100ul well suspended cells in eppis and freeze immediately in lN2. Store at -80C SOB 500ml (use within 2-3 weeks) Bactotryptone
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