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文献和实验Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
/negative genes can be introduced to study the effect of signal interference on cells. This basic protocol is concerned with the application of GFP as an indicator of transfection in chicken embryos. Lipofectin® Reagent (Life Technologies, catalog
Transient Transfection of Cos-1 Cell
to, cell density, transfection reagent, duration of transfection, and DNA concentration.1. Seed a six-well tissue culture plate with 1-2 x 105 Cos-1 cells in 2ml of DMEM supplemented with 10% fetal calf serum. Incubate for 18-24 hours at 37¼C in a CO2
Transient Transfection of Cos-1 Cells
PLUS Reagent kit (Life Technologies). Allow mixture to stand for 15 minutes at room temperature. In a second tube dilute 4 microliters of LIPOFECTAMINETM Reagent into 100 microliters serum free DMEM. 3. Combine the two solutions, mixing gently and incubate
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