epigentek特约代理商|P-2013-48 EpiQu

Chromatin Immunoprecipitation (ChIP)

of formaldehyde in the ChIP method was pioneered by Solomon and Varshavsky (Solomon et al. 1988). In their original studies, the association of histone H4 and RNA polymerase II with the Drosophila hsp70 genes was monitored. Cells were treated with formaldehyde

Whole genome amplification protocol for ChIP-chip

protocols for ChIP assays from mammalian cells and tissue samples, as well as preparation of amplicons can be found on the Farnham Lab website. Procedure The Whole Genome Amplification using Sigma GenomePlex Kit was adapted from the manufacturer's

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained