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上海玉博生物科技有限公司
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文献和实验of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately and ready to use in any downstream application. The kit is also available in spin-column format for micro- and mini-sized samples. Principle and procedure
Purification of Genomic DNA Using PureLink Silica Columns
each to obtain higher DNA yield. The DNA recovery in the first elution is 65-80% and after second elution is >95%. To prevent dilution of the DNA sample and also avoid contact of the spin column with the eluate, perform the two-elution steps using
-chloroform extraction, b) Using kit column, a) Phenol-chloroform extraction: Materials: Saturated phenol, chloroform, 3 M sodium acetate pH 5.2 or 5 M ammonium acetate, 100% ethanol or isopropanol. Methods: 1. Add equal volume of saturated
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