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文献和实验Printing Low Density Protein Arrays in Microplates
were simultaneously analyzed without detectable cross-reactivity associated with capture antibody or secondary antibody interactions. Detection of interleukin antigens, spiked into cell culture media containing 10% fetal calf serum, was specific and sensitive.
Evaluating a Screen and Analysis of Mutant Libraries
individual colonies to multi-well microplates containing culture medium, growing the cells until stationary phase, and inducing protein expression. If the target protein is intracellular, a cell lysis step has to be carried out to release the cellular
High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins
microplates from which they are eluted after washing. A 1.5-mL cell culture typically yields 5-10 pmol recombinant protein.
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