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Study of G Protein-Coupled Receptor/-arrestin Interactions Within Endosomes Using FRAP

of β-arrestin with a GPCR, the bradykinin type 2 receptor (B2R). We provide a detailed protocol to assess the avidity of β-arrestin2-YFP for B2R within endosomes in HEK293 cells. β-arrestin2-YFP associated with internalized receptors is photobleached

Rho1D4纯化体系：专为重组膜蛋白提取设计

1：膜蛋白应用实例 蛋白表达系统纯度产率 （所有回收蛋白）采取的纯化步骤hCB2 G蛋白偶联受体大肠杆菌90%4.5mg/5L 培养物Rho1D4 IAC+SEChVN1R1 G蛋白偶联受体可诱导的HEK293S细胞系（哺乳动物）90%1mg/1g细胞Rho1D4 IAC+SECFPR3 G蛋白偶联受体可诱导的HEK293S细胞系（哺乳动物）90%2mg/6g细胞Rho1D4 IAC+SECTAAR5 G蛋白偶联受体可诱导的HEK293S细胞系（哺乳动物）90

Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters

in intracellular Ca2+ levels and PKC translocation to the plasma membrane in response to mGluR1 stimulation in transiently transfected human embryonic kidney (HEK293) cells. The changes in intracellular Ca2+ were imaged using the fluorescent indicator Oregon Green