Fitzgerald特约代理商|55R-1039 FACT

Identification of a Mutant Kinase/ATP Analog Pair

(if [y-32 P]ATP analog is used) or immunoblotted with a phosphospecific antibody (if available) to determine the extent of substrate phosphorylation. The kinase/ATP analog pair that provides the best phosphorylation of the substrate is then used

mTn-3xHA/GFP

) or MMS101R (BAbCo, Richmond, California). These antibody recognise cross-reacting yeast proteins of about 55kD or110kD, respectively, and can give a spotty background on immunofluorescence. Despite this drawback, the 3xHA tag has been used extensively

核蛋白基因组指纹技术Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells

, pepstatin; each final concentration 2 µg/ml) Protein A/G agarose beads   Antibody   Dilution buffer (stable at room temperature; add PMSF and proteinase inhibitors and put on ice before use) 10 mM Tris-HCl (pH 8.0)