illumina特约代理商|FC-404-2003 Next

Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer

DEPC‐treated H 2 O ( appendix 2D ) 10 µM GEX Adapter 1 (from Illumina Tag Profiling Sample Prep Kit, cat. no. FC‐102‐1005

Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells

, NTPs, buffer, Mg++ ) Phenol/chloroform/isoamyl alcohol Protein A/G agarose beads (50%), pre-swollen and blocked (Santa Cruz, sc-2003) Proteinase K (20 mg/ml; store at -20°C) Random-primed DNA synthesis kit RIPA buffer

A Proteomics Approach to Identify Redox-Sensitive Proteins

concentration is determined with the CB-X Protein Assay kit. 3. Protein Extraction and Labeling: For Blocking Method    1) Five hundred microliters of cells are broken in 1.5-ml Eppendorf tubes with plastic micro-pestle on ice in 500 ml