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- Bertin
- D34010
- 2025年07月14日
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上海玉博生物科技有限公司
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文献和实验monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
Polyclonal and Monoclonal Antibodies
) Leave 1 hr at room temp or O.N at 4¡C. 5) SUBSTRATE a) Disolve substrate in water. b) Wash plate 4 times with PBS + 0.1% Tween 20 (use TBS instead of PBS for AP). c) Add 100 ul of substrate to every well. d) Watch color development
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