fluka特约代理商|17836-50G Ammonium

Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis

volume of Chloroform, mix well by inversion, centrifuge in a microcentrifuge at full speed for 3 min to separate the phases. Save the aqueous phase (upper layer). 4. To precipitate the DNA, add 0.1 volumes of 3 M Ammonium Acetate and 2.5 volumes of 100

Silver Enhancement Protocol

for microbial contamination before each use if not making fresh each time. 2. Solution A: Dissolve 100 mg silver acetate (Fluka Chemical Co., catalog # 85140) in 50 ml distilled water and keep under cover. To speed dissolving the fluffy powder, stir

Transformation of E. coli by Electroporation

  Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate