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上海玉博生物科技有限公司
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文献和实验Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides
) Reversed‐phase columns: e.g., Lichrospher 100 RP 18 (5 µm, 125 × 4 mm, or 10 µm, 250 ×10 mm; Merck) or CC Nucleosil 100‐5 C18 (125/4) column (Macherey‐Nagel) Desalting
NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGENIN dUTP
solution + 27 µl bidest (1) 25 µl of each (1) +25 µl H20 = 100 µl mix 2.5 µl bio-16-dUTP (0.4 mM, ready for use) 5 µl 100 mM DTT x µl DNA (usual 1 mg disolved in 1 ml TE) 5 µl DNase I (stock
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
DTT Superscript II RT (Gibco) RNasin (Gibco, optional) 0.1M 200U/mL 40U/mL 6.0 mL 0.6 3.0 1.5 0.5 50X dNTP stock solution using a 4:1 ratio aminoallyl-dUTP to dTTP***: 10 mL each 100 mM
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