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文献和实验.27. Column Buffers Make 500mls equivalent of base buffer: 10ml 1M Hepes pH 7.9 100ml 10% glycerol 5ml 10% NP40 (or 5.0ml 300mM Octyl glucoside) 200ml 0.5 EDTA ------- 115.2ml /5 = 23ml Base 1M KCl2.5M KCl dH20 for 0 KCL 46ml 0 0 154ml = 200
Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
a minimum of 4.0 ml of each buffer per 100 ml of culture regardless of which cartridge size is being used. However, if the particular cartridge requires more than this, then use the prescribed amount for the cartridge. For example, if a 100 ml BAC culture
the RNA to the cellulose and mix on rotator for 1 hour at room temperature.6. Prepare a BIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008） by washing it out once with 750 l 1X NETS.7. Gently pour the RNA/cellulose mixture into the column
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