swift特约代理商|23048 Accel-NGS 2S

freeshotgun sequencing library from miniprep BAC DNA

extraction kit. In the last step, use 30 μl of EB( elution buffer) to elute DNA from column and collect ~ 28 μl DNA. 11.Ligate Ligate size selected DNA fragments into subvectors using Ready-To -Go TM pUC18 Smal1/BAP Ligase ( Phamarcia Biotech

free shotgun sequencing library from miniprep BAC DNA

with gelase ( Gelase TM Agarose Gel-Digesting Properation, Epicentre Technologies, #09200) or QIAquick Gel Extraction Kit ( Qiagen, # 28704) , or GeneClean Kit for Purification of DNA from agarose gel ( Bio 101, #3105). 9.Sonication and end-repair DNA

DNA SMART技术让ChIPSeq研究更轻松

一代测序（NGS）文库就是科研人员面临的挑战之一。大部分ChIP测序文库的制备需要测序接头连接，但是这些制备方法的起始材料仅限于双链DNA。DNA SMART ChIP-Seq Kit提供了一种强大、可靠的DNA ChIP-seq方法，尤其适用于低起始量材料（100 pg–10 ng），整个文库制备过程只需4小时，不仅适用于dsDNA也适用于ssDNA，是DNA CHIP-Seq的理想选择。DNA SMART技术DNA SMART技术传承了SMART技术的优势，无需接头连接和纯化，整个过程依赖