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AllPrep DNA/RNA 96 Kit

using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates

SequalPrep Long PCR Kit with dNTPs

Volume Final Concentration SequalPrep™ 10X Reaction Buffer 2 μl 1X DMSO

FFPE Direct PCR Kit

for 60 minutes.    5) Add 100 μl FP3 Buffer and vortex to mix.    6) Store the extraction at 2-8°C. 2. PCR Protocol This protocol serves as a guideline for PCR amplification. Optimal reaction conditions, such as incubation times