ABI 特约代理商|750023 UltraPure™ DE

RNase and DEPC Treatment: Fact or Laboratory Myth

effects. Template DNA was dried to completion in a vacuum centrifuge and resuspended in 0.01%, 0.1% or 1% DEPC-treated water. Duplicate MAXIscript™ transcription reactions were assembled using 32 P-UTP, and the same concentration of DEPC treated water

Synthesis of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water

evaporator equipped with a water aspirator or vacuum pump Automatic DNA synthesizer (e.g., ABI 3400, Applied Biosystems)

MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

centrifuge. Total RNA was precipitated with 2 vol of 2-propanol (Fluka) at −20°C for at least 16 h. The RNA pellet was washed with 75% ethanol (Merck), dried, and dissolved in DEPC-treated water (Ambion). The RNA was quantified with Quant-iT™ RiboGreen® RNA