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Activation of Nuclear Factor-B

Nuclear factor-κB (NF-κB) is a key transcription factor that regulates the expression of genes involved in immune and inflammatory responses and in cell death and survival. This chapter describes in detail the method for measuring the NF-κB

Purification, Quantification, and Functional Analysis of Complement Factor H

Complement Factor H (FH) is an abundant, non-enzymic plasma/serum glycoprotein, which has a major role in regulating activation of the complement system. It can be purified from human plasma/serum by affinity chromatography, using

Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells Using Flow Cytometry

The complement receptor 2 (Cr2 ) gene is exclusively expressed in B cells and follicular dendritic cells (FDC) in mice and in humans. However, mice also express an alternative splice variant, CR1, of the Cr2 gene. CR2 and CR1 are receptors