gibco特约代理商|10439024 Fetal Bovi
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文献和实验Isolation and Culture of Bovine Embryonic Stem Cells
Isolation and culture of primary embryonic stem (ES) cell colonies are the first critical step towards establishment of stable ES cell lines. Here we introduce a novel method designated as “Separate and Seed” that contributes remarkably
Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells
. However, hESCs are usually grown in culture conditions using fetal bovine serum and mouse embryonic fibroblasts, two components that are not compatible with clinical applications. Consequently, the possibility to expand hESCs in serum-free and in feeder-free
Primary Cultures of Astrocytes from Fetal Bovine Brain
We describe here a method to obtain primary cell cultures from the cerebral cortex and the hypothalamus of bovine fetuses. We report how tissue origin, developmental stages, and culture medium conditions influence cell
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