Novus特约代理商|NB500-452F Endoglin

流式细胞技术测定NF-kappaB的转移

at 4 °C with FITC-conjugated goat anti-mouse anti-Ig antibody fragments (1/50; DAKO Glostrup, Denmark). After washings in PBS–FCS, nuclei were analysed by flow cytometry on a FACSvantage SE (BD Biosciences, San Jose, CA, USA) equipped with the pulse

Intracellular Cytokine Staining Protocol

anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified

【讨论】抑制免疫到底是好还是坏?

29 (MEM 101A, purified IgG1) was used as reported previously [9–11]. FITC-labeled antibodies to CD80, CD86, CD29, and CD18 were obtained from Immunotech (Marseilles, France).2.2. AnimalsC57BL/6 male mice (6–8 weeks old, 17–21 g) were obtained from DAEHAN