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文献和实验RACE System for Rapid Amplification of cDNA Ends
amplification by PCR. Control RNA, DNA, and primers are provided for monitoring system performance. First strand cDNA is synthesized from total or poly(A) RNA using a gene-specific primer (GSP1) that the user provides and SuperScript™ II, a derivative
Dynabeads® mRNA DIRECT Micro Kit - mRNA isolation for RT-PCR amplification
实验原理 Use the Dynabeads® mRNA DIRECT™ Micro Kit for simple and rapid isolation of mRNA from small cell and tissue samples, for direct use in reverse transcription PCR. The direct mRNA isolation is completed in only 15
are PCR products that include promoter and terminator sequences flanking a hairpin siRNA template and can be prepared with Ambion's Silencer™ Express Kits. This screening strategy also permits the rapid identification of the best combination of promoter
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