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文献和实验monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
until examination. Antibodies mouse monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used
Western Blotting with Monoclonal Ant
the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies). 3.Decant the wash buffer, add the diluted enzyme conjugate and incubate with agitation for 30 minutes
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