AMBion特约代理商|AM9261 EDTA (0.5 M

0.5M EDTA

0.5M EDTA   FW M L pH8.0 xg = 292.25 0.5 0.5   **93.06g** Add to about 300mls H2 O while spinning

Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer

Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij

高温处理＋柠檬酸钠或EDTA修复石蜡包埋样品抗原

. Incubate slides in DAB or other suitable peroxidase substrate. 17. Wash thoroughly in running tap water. 18. Counterstain with haematoxylin (if required), dehydrate and mount. SOLUTIONS 1. 0.01M CITRATE BUFFER (pH 6.0) Add 3.84 grams of citric