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Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I

System. 9.  Purification of ssRNA was carried out following the protocol described in the section III-E of the Instruction Manual of the MEGAscript® RNAi Kit (Ambion, USA

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

containing chloramphenicol (Cm, 20 μg/ml)/streptomycin (Stp, 75 μg/ml) and purified using the Large construct kit (Qiagen, Valencia, CA, USA). PCR PCR reactions using oligonucleotides carrying HAs were performed using

Recombineering‐Based Procedure for Creating BAC Transgene Constructs for Animals and Cell Lines

Gel purification kit (Qiagen) Safe Imager blue‐light transilluminator (Invitrogen)