qiagen特约代理商|51106 QIAamp DNA B

DNA Purification from Blood or Body Fluids

QIAGEN Protease (or proteinase K) and 400 µl Buffer AL. If sample volumes larger than 400 µl are required, use of QIAamp DNA Blood Midi or Maxi Kits is recommended; these can process up to 2 ml or up to 10 ml of sample, respectively. Note

E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol

6-7 times. Incubate at room temperature for 1-2 minutes. 9. Transfer 700 u of the mixture(from Step 8) into a clean HiBindTM DNA Mini Column assembled in a 2 ml collection tube(provided). Centrifuge at 10,000 × g for 1 min at room temperature to pass

E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol

with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times