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文献和实验), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
). 9. Secondary antibody to use with goat polyclonal reagent: donkeyanti-goat IgG-horseradish peroxidase (HRP) (sc-2056;Santa Cruz Biotechnology). 10. Secondary antibody to use with rabbit polyclonal reagent: donkey anti-rabbit IgG-HRP (#NA934V, GE
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