- ￥3000
- Accutase（Innovative Cell Technologies
- AT104
- 2025年07月11日
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上海玉博生物科技有限公司
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文献和实验-cell suspension. If you removed adherent cells by trypsinization, etc., resuspend them well (observe under microscope). Cell clumps should also be removed by passing cells through a cell-strainer. Note that there are reagents such as Accutase™
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
Recombinant human FGF basic (R&D Systems, Catalog # 233-FB , 4114-TC , or equivalent) Accutase (Innovative Cell Technologies, Catalog # AT104 or equivalent) Cultrex® Basement Membrane Extract (BME) (R&D Systems, Catalog # 3433-005-01 or equivalent
before splitting and decide whether to adjust recommended dilution factor accordingly. A. Semi-adherent cell lines - will stick to new flask but come loose over time S2* 3-4 days 1:3 - 1:4 S
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