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文献和实验ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
ECL Millipore WBKLS0500 Methanol (dried) Sinopharm 10014118 nacl Sinopharm 10019392 goat anti rabbit IgG（H+L），HRP Jackson 111-035-003 goat anti mous IgG（H+L），HRP Jackson 115
Incubate the plate overnight at 4°C (or for 2-3 hr at 30°C). Wash the plate extensively (3 x 5 min) with 300 µl/well of HBST. Dilute goat anti-rabbit IgG coupled to AP 5,000-10,000
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