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文献和实验Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
profiling addresses the methylated sequences (methylome) of patient genomes to identify disease-specific methylation patterns. Workload in methylome analyses can be considerably reduced by methylome enrichment using proteins or antibodies with high affinity
Methylated DNA Immunoprecipitation (MeDIP)
procedure generally yields 5% of the original total DNA in mammalian cells (i.e. 200 ng of methylated DNA starting from 4 µg total DNA). Back to top Reviewer Comments Reviewed by: Daniel Zilberman, University of California, Berkeley, CA
Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA
I provide a protocol for DNA methylation profiling based on immunoprecipitation of methylated DNA using commercially available monoclonal antibodies that specifically recognize 5-methylcytosine. Quantification of the level of enrichment
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