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文献和实验Phosphoamino acid analysis:Mark Kamps's method
of PAA standards.15 min should be plenty. 13.Mark the plate with radioactive ink so that you can extrapolate where the origins were and can align the film unambigously with the plate and the PAA markers. 14.Expose the plate to flashed film with a screen
Introduction of human 14-3-3 proteins
protein kinase II,regulation of protein kinase C,phospholipase A2 activity,stimulation of exocytosis and activation of bacterial exoenzyme S (ExoS)during ADP-ribosylation of host proteins.In addition,a plant 14-3-3 protein is present in a G-box DNA/protein
off the EtOH and let pellet air dry （or speed vac dry on low heat）. 14. Resuspend in 15-20 ul RNase-free water. 15. Spin down for 30 seconds and quantitate a 1:100 dilution at 260nm. Note: Typically yields should be no greater than 2% of the total RNA input
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