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文献和实验Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
Wash buffer (see recipe ) 20 µg each of the bait proteins at 400 ng/µl (see protocol 2 ) Dilution
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Immunohistochemisty-Fluorescence Protocol
temperature with 15 µL of flurochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1 or IgG2A or IgG2B diluted 1:300) in Wash Buffer-Saponin supplemented with 5 human AB serum. Wash slides three times in Wash Buffer-Saponin and air
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