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文献和实验-His fusions)or 900 ml (MBP fusions)LB medium (Sambrook et al.,1989)containing kanamycin at 50 µg/ml (the plasmid vector confers resistance to this drug).The cultures were shaken vigorously at 37ºC until the optical density at 600 nm reached 0.5; IPTG
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
REFERENCES Cox, G.N., Pratt D., McDermott M.J. and van der Slice R.W. 1999. Refolding and characterization of recombinant human soluble CTLA-4 expressed in E. coli. Protein Expr. Purif . 17 : 26-32. Frangoni J
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
/ml Leupeptin (stock 10 mg/ml in DMSO) 10 µg/ml Pepstatin (stock 10 mg/ml in DMSO) 0.1 mM PMSF (stock 100 mM in isopropanol) 0.2 mM Sodium Vanadate (stock 0.1 M in H2 O) 5 mM Sodium Flouride
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