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BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod

into 50 ml Corning centrifuge tube and DNase-free RNase A is added to a final concentration of 100 ug/ml from a 20 mg/ml stock and RNase T1 is added to a final concentration of 40 ul/100 ml from a 100 U/ul stock followed by incubation in a 37 deg C water

ES-200A电子天平使用说明

的情况下按“校正”(C)键，显示窗出现“CAL”进入校正状态，再将标准砝码放于台上，待稳定后天平显示标准砝码重量值，并出现重量符号“g”后校正即告完毕，可进行正常称量。 4、如被称物件重量超出天平称量范围，天平将显示“Err--H”以示警告。 5、如需去除器皿皮重，则先将器皿放于称台上，待示值稳定后按“去皮”(T)键，则天平显示“0”，然后将需称重物品放于器皿上，此时显示的数字为物品的净重，拿掉物品及器皿，天平显示的负值，仍按“去皮”使显示回到“0”。 6、计数功能的使用  

BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method

. Then, the supernatant of each tube is transfered into 50 ml Corning centrifuge tube and DNase-free RNase A is added to a final concentration of 100 ug/ml from a 20 mg/ml stock and RNase T1 is added to a final concentration of 40 ul/100 ml from a 100 U/ul stock followed