Biolegend特约代理商|100328 PerCP/Cy

Western Blot/Anti-P-CREB

mm dish. This is about 100 ug protein for PC12 cells.   After electrophoresis, electrotransfer to nitrocellulose membrane. I include in the SDS-PAGE prestained molecular weight markers to check the efficiency of the transfer

Transgenic Mouse Core Facility

1.Restrict DNA and gel purify Cut 100ug DNA, removing as much plasmid sequence as possible from the insert. Run on an agarose gel. Cut out band and electroelute in 0.5X TBE. Add an equal volume of Tris saturated phenol with b

Mouse Tail DNA Extraction

  1. Cut 0.5 cm of tail and mark mouse ears or toes   2. Incubate at 55°C O/N in:   500 l Tail Extraction Buffer 10 l Proteinase K (10 mg/ml) 12.5 l 20% SDS   3. Add 150 l 5 M NaCl