Anti-SELPLG Polyclonal Antibod

产品简介

单位	支
英文名称	Anti-EGLN3 Polyclonal Antibody
别名	PHD3;HIFPH3;HIFP4H3
宿主	Rabbit
稀释比例	WB 1:200-1000.
Gene ID	112399
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	Q9H6Z9
蛋白分子量	27kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
免疫原	Recombinant protein of human EGLN3
亚型	IgG
性状	液体
纯化方法	Affinity purification
背景资料	Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis.
Public Immunogen Range	Recombinant protein of human EGLN3
克隆类型	Polyclonal Antibody
交叉反应	Human Rat Mouse
应用	WB
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Western blot analysis with EGLN3 antibody diluted at 1:200; Lane:Mouse heart