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文献和实验Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells
that is mRNA derived. The cDNA pools can be used directly for gene-specific reverse transcriptase polymerase chain reaction or processed for ligation into vectors of choice whereas the amplified RNA can be used for microarray-based expression profiling.
Isolation of Coding Sequences from Genomic Regions Using Direct Selection
The rate-limiting steps in the positional cloning of disease genes are the identification of discrete genes within a large genomic region and the analysis of mutations in these genes. Direct selection is an expression-based gene
of their current sequencing machine, the GSFLX. The system relies on fixing nebulized and adapter-ligated DNA fragments to small DNA-capture beads in a water-in-oil emulsion. The DNA fixed to these beads is then amplified by PCR. Finally, each DNA-bound bead
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