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文献和实验A general protocol for staining cell for cytometry analysis
according to cell type, and stage of apoptosis. In some cases, investigators may also need to reduce the number of cells to 0.5 x 10 5 /100 µl and still add 5-15 µg of recombinant Annexin V to obtain optimal results. 4. Gently mix the cells
The Easiest Route to Guaranteed Silencing
The Easiest Route to Guaranteed Silencing Increasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induced RNA interference (RNAi) include
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
DETECTION OF ß-GALACTOSIDASE The E. coli lacZ gene encoding ß-galactosidase (ß-gal) is the classical
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