Fig 1. Western blotting by primary antibody rat anti L1 (Millipore Corp, cat. # MAB5272) and secondary antibody goat anti rat, HRP (Millipore Corp, ca...
Description:
Neurite growth protein L1
Trade Name:
Chemicon (Millipore)
Background Information:
L1, a cell adhesion molecule of the Ig superfamily (IgCAM) plays a critical role in the formation of neuronal networks. This is reflected by the variety of clinical signs associated with the X-linked recessive neurological disorder that is caused by mutations in the L1 gene. L1 regulates the formation of axon fascicles and promotes neurite outgrowth through interaction with a wide spectrum of binding partners including cell adhesion molecules and extra-cellular matrix components. L1 participates in the signaling of a secreted guidance cue of the Semaphorin family, Sema3A. Three types of experimental evidence support L1 as a key component of the Sema3A receptor complex.
Chimeric protein of Human neurite growth protein L1 and Human IgG-Fc γ domain purified using protein A-agarose (Millipore Corp, cat. # 16-125). MW ~ 200 kDa.
Application Notes:
Used at 5 μg/ml (see references 1 and 2).
Optimal working concentration must be determined by end user.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Source:
Mammalian cells, HEK293 cell line
Presentation:
100 µg of recombinant protein in 100µl of 1X PBS, 20%(v/v) glycerol. Frozen solution.
Storage Conditions:
Shipped on dry ice. Best stored at -80 C for up to 6 months after date of receipt. Thawed aliquots should be used immediately, as repeated freeze/thaw cycles might result in loss of activity.
Specific Activity:
See references.
Protein or Enzyme Type:
Neurobiology
Recombinant Protein
Concentration:
1 mg/mL
Purity:
Greater than 90% by SDS-PAGE and Coomassie blue staining.
Fig 1. Western blotting by primary antibody rat anti L1 (Millipore Corp, cat. # MAB5272) and secondary antibody goat anti rat, HRP (Millipore Corp, cat. # AP183P). Single band expected at ~ 200 kDa