Anti-Muscarinic Acetylcholine Receptor m1; 200 μL产品图

Anti-Muscarinic Acetylcholine

Receptor m1; 200 μL
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  • 询价
  • AB5164-200UL
  • 2025年12月31日
  • Western Blotting;Immunohistochemistry
  • Rabbit
  • Mouse;Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      Western Blotting;Immunohistochemistry

    • 宿主：

      Rabbit

    • 适应物种：

      Mouse;Rat

    • 库存：

      大量

    • 抗原来源：

      0

    • 是否单克隆：

      2

    • 规格：

      200 μL

    Product Images

    Western blotting of total rat brain membrane proteins (50 mg/lane)

    1. AB5164 1:200

    2. AB5164 preincubated with control fusion ...

    Rabbit anti-Muscarinic Acetylcholine Receptor m1 (Catalog Number AB5164) staining of rat cortex neurons.
    Species Reactivity Key Applications Host Format Antibody Type
    M, R IH, WB Rabbit Affinity Purified Polyclonal Antibody
    Description:
    Anti-Muscarinic Acetylcholine Receptor m1
    Specificity:
    Recognizes a full length m1 protein. It has exhibited no cross reactivity with other muscarinic proteins tested so far.
    Key Applications:
    • Immunohistochemistry
    • Western Blotting
    Application Notes:
    Western blot: 1:200 using ECL on rat brain membranes with proteinase inhibitors. M1 is approximately 60-80kDa in size, some preparations yield 125kDa dimers (photo). Heating samples to 80-90C rather than fulling boiling and adjusting sample pH to neutral can help in preventing dimer formation.

    Dilutions should be made using a carrier protein such as BSA (1-3%)

    Immunohistochemistry: rat frozen sections. 1:50-1:200, internal epitope; use triton X-100 in blocking buffer only; dilute primary antibody in PBS or TBS with 0.5%-1% BSA or NGS only.



    Optimal working dilutions must be determined by the end user.
    Species Reactivity:
    • Mouse
    • Rat
    Immunogen:
    GST fusion protein and part of i3 intercellular loop of human m1 muscarinic acetylcholine receptor (amino acids 227-353) (Accession P11229).
    Control:
    Included free of charge with the antibody is 60 μg of control fusion protein (lyophilized powder). The stock solution of the fusion protein can be made up using 100 μL of PBS. For positive control, in Western blot using 10 ng of protein per minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    Presentation:
    Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
    Storage Conditions:
    Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    Gene Symbol:
    • CHRM1
    • M1
    • HM1
    • MGC30125
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    Neurotransmitters & Receptors
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Therapeutic Areas:
    Oncology
    Format:
    Affinity Purified
    Host:
    Rabbit
    Concentration:
    0.8 mg/mL (after reconstitution)
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    GST fusion protein and part of i3 intercellular loop of human m1 muscarinic acetylcholine receptor (amino acids 227-353) (Accession P11229). 详细描述见链接：http://www.millipore.com/catalogue/item/AB5164-200UL

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    图标文献和实验
    相关实验

    • Solubilized Muscarinic Acetylcholine Receptors from the Rat Myocardium: Pharmacological and Hydrodynamic Characterization

      The vast majority of receptors are membrane proteins, so solubilization is an essential prerequisite for purification of these receptors. If the receptor can be solubilized without destroying its characteristic ability to bind specific

    • Muscarinic Cholinergic Receptors

      receptor population is divided between nicotinic and muscarinic subclasses, the CNS-associated cholinergic receptor appears to be predominantly of the muscarinic type, although there is evidence that the spinal cord contains substantial amounts of nicotinic

    • Overview of Receptor Allosterism

      Figure 1.21.1 Allosterism between a receptor and its G protein: the effects of GTP on the competition between [3 H] N ‐methylscopolamine and the agonist, carbachol, at the muscarinic acetylcholine M2 receptor in rat myocardial membranes. Competition

    查看更多相关实验 >
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