Human CCL17/TARC Quantikine EL

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
7 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human TARC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human TARC Immunoassay kit is a 3.5 hour solid phase ELISA designed to measure TARC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human TARC and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TARC. Results obtained using natural human TARC showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TARC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	87	600	1107	98	616	1178
Standard Deviation	6.2	13.7	29.9	8.9	50.7	88.8
CV%	7.1	2.3	2.7	9.1	8.2	7.5

 

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	88	504	1001	89	540	1033
Standard Deviation	4.1	21.7	27.2	8.3	38.9	71.7
CV%	4.7	4.3	2.7	9.3	7.2	6.9

 

Recovery

The recovery of TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.

 

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	95	88-104
EDTA Plasma (n=5)	102	86-111
Heparin Plasma (n=5)	98	86-107
Serum (n=5)	105	100-114

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of TARC were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Data Examples

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Human CCL17/TARC ELISA Calibrator Diluent RD5-5 Standard Curve