EmbryoMax® ES Cell Qualified 2
X Cell Culture Freezing Medium, Formulated with 20% DMSO & Fetal- 询价
- ES-002-D
- 2025年07月15日
- Stem Cell Culture
- 0
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- 详细信息
- 技术资料
- 应用范围：
Stem Cell Culture
- 宿主：
0
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
0
- 规格：
50 mL
Description:
EmbryoMax® ES Cell Qualified 2X Cell Culture Freezing Medium, Formulated with 20% DMSO & Fetal Bovine Serum
Product Overview:
This unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data)and is ES cell qualified. Freezing Media contain 10% of the active cryopreservation ingredient (either DMSO or Glycerol). The remaining 90% is a proprietary mixture of Calf and Fetal Bovine Serum. The reagents used in these products are certified to be virus and mycoplasma free.
The sera utilized in this medium is approved by the United States Department of Agriculture (USDA) to ensure that sera is free of diseases such as Bovine Spongiform Encephalopathy (BSE).
The sera utilized in this medium is approved by the United States Department of Agriculture (USDA) to ensure that sera is free of diseases such as Bovine Spongiform Encephalopathy (BSE).
Key Applications:
Stem Cell Culture
Application Notes:
Picking and freezing of ES cells:
Wash Plate with PBS. Pick colonies into 96 wll plate (U shaped wells) with 35 µl of trypsin / well. Pick roughly 48 colonies, incubate at 37 °C for 10 minutes. Use Multichannel pipet to break up colonies. Transfer directly into 96 well plate with feeders and 150 µl of ES medium (without selection drug). Feed every day with ES medium containing selection drug (150 µg /ml neomycin, or 50 µg /ml of hygromycin). After 2 days cells should be ready to be frozen. Wash with PBS add 35 µl of Trypsin, incubate at 37 °C for 10 minutes, add 65 µl of ES medium. Use multichannel pipet to disperse cells. Transfer 65 µl into replica plate (U shaped wells) containing 65 µl of cold 2 x ES freezing medium. Wrap with parafilm, place on dry ice for 20 minutes, and transfer to -80 °C.
Wash Plate with PBS. Pick colonies into 96 wll plate (U shaped wells) with 35 µl of trypsin / well. Pick roughly 48 colonies, incubate at 37 °C for 10 minutes. Use Multichannel pipet to break up colonies. Transfer directly into 96 well plate with feeders and 150 µl of ES medium (without selection drug). Feed every day with ES medium containing selection drug (150 µg /ml neomycin, or 50 µg /ml of hygromycin). After 2 days cells should be ready to be frozen. Wash with PBS add 35 µl of Trypsin, incubate at 37 °C for 10 minutes, add 65 µl of ES medium. Use multichannel pipet to disperse cells. Transfer 65 µl into replica plate (U shaped wells) containing 65 µl of cold 2 x ES freezing medium. Wrap with parafilm, place on dry ice for 20 minutes, and transfer to -80 °C.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Media or Reagent Type:
Freezing Media
Stem Cell Type:
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
- Maintenance & Expansion
- General Cell Culture
Media Type:
Cell Culture Freezing Media
Storage Conditions:
Store at -20°C upto 2 years
Cell Culture Reagent Type:
- Cell Culture Supplements
- Stem Cell Reagents
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/ES-002-D
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EmbryoMax® ES Cell Qualified 2X Cell Culture Freezing Medium, Formulated with 20% DMSO & Fetal
询价
