Immortalized Mouse Kupffer Cel

Immortalised Mouse Kupffer Cells (ImKC) are isolated from male H-2Kb-tsA58 transgenic mice which expresses the thermolabile mutant tsA58 of SV40 40 large T antigen and were sorted through identifying the F4/80 marker. The Immortalised cells expresses p53 levels with increases telomerase activity and retains the some functional features compared to the primary cells. ImKC cells can propagate without the presence of IFNy at 37°C, and are able to produce proinflammatory cytokines when induced with lipopolysaccharide (LPS). These cells are valuable for studies in proinflammatory cytokines (TNF-α, IL-6 and IL-1β) and their roles in Kuppfer cells, in processes in liver injury, and for research in chronic diseases.从表达SV40 40大T抗原的热稳定性突变体tsA58的雄性H-2Kb-tsA58转基因小鼠中分离得到ImKC细胞，并通过鉴定F4/80标记进行分类。与原发细胞相比，永生细胞表达p53水平，端粒酶活性增加，并保留了一些功能特征。在没有IFNy的情况下，ImKC细胞在37c可以增殖，在脂多糖(lipopolysaccharide, LPS)的诱导下可以产生促炎细胞因子。这些细胞对于研究促炎细胞因子(TNF- TNF、IL-6和IL-1细胞因子)及其在Kuppfer细胞中的作用、在肝损伤过程中的作用以及慢性疾病的研究都有价值。