Cultures of dissociated NSCs were generated from human fetal brain tissue of 10-14 weeks gestation. NSCs were further cultured under low oxygen conditions and were transduced with retrovirus carrying L-MYC gene (LM-NSC008).Neural stem cells (NSCs) can limit or reverse CNS damage through direct cell replacement, promotion of regeneration, or delivery of therapeutic agents. Immortalised NSC lines are in growing demand due to the inherent limitations of adult patient-derived NSCs, including availability, expandability, potential for genetic modifications, and costs.从妊娠10-14周的胎儿脑组织中提取分离的神经干细胞。在低氧条件下进一步培养NSCs,并将其转化为携带L-MYC基因的逆转录病毒(LM-NSC008)。神经干细胞(NSCs)可以通过直接替换细胞、促进再生或提供治疗药物来限制或逆转中枢神经系统的损伤。由于成人患者源性神经干细胞的固有局限性,包括可用性、可扩展性、基因改造的潜力和成本,永生性神经干细胞系的需求不断增长。