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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
零下80~20°C
- 保质期:
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名:
2-(2-bromophenyl)-5-chloro-4H-3,1-benzoxazin-4-one
- 库存:
10
- 供应商:
TargetMol
- CAS号:
1234707-32-4
- 规格:
1 mg/50 mg/5 mg/100 mg/25 mg/500 mg/2 mg/1 mL/10 mg
| 规格: | 1 mg | 产品价格: | ¥774.0 |
|---|---|---|---|
| 规格: | 50 mg | 产品价格: | ¥5590.0 |
| 规格: | 5 mg | 产品价格: | ¥1750.0 |
| 规格: | 100 mg | 产品价格: | ¥7500.0 |
| 规格: | 25 mg | 产品价格: | ¥4130.0 |
| 规格: | 500 mg | 产品价格: | ¥15100.0 |
| 规格: | 2 mg | 产品价格: | ¥1150.0 |
| 规格: | 1 mL | 产品价格: | ¥1720.0 |
| 规格: | 10 mg | 产品价格: | ¥2590.0 |
Product Introduction
Bioactivity
英文名:2-(2-bromophenyl)-5-chloro-4H-3,1-benzoxazin-4-one
描述:2-(2-bromophenyl)-5-chloro-4H-3,1-benzoxazin-4-one is an human neutrophil elastase inhibitor.

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文献和实验deionized formamide 500 m l 10X TBE 500 m l 0.2% bromophenyl blue and 0.2% xylene cyanol Other Reagents Needed: 0.22 m M disposable syringe filters, short wave UV source, intensifying screen for UV shadowing Procedure • Pour a 20% denaturing gel: 12 ml
Protein Gel Preparation and Staining
250 mM Tris Base 1.65 g Tris Base 250 mM glycine 9.35 g glycine 0.1% SDS 2.5 ml 20% SDS up to 500 ml with Q 5X Loading Dye 0.5 M Tris 6.8 5 ml 1M Tris pH 6.8 50% glycerol 5 ml glycerol 0.5% bromophenyl blue 0.05 g
In vitro transcription with yeast nuclear extract
). Incubate 3 min at room temp. 6. Add 3 microliters sequencing gel loading buffer (formamide/EDTA plus bromophenyl blue and xylene cyanol) and mix well. 7. Heat 1 min at 90 - 100 degrees and immediately put on ice. 8. Load to 6% DNA sequencing gel and run
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