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- 详细信息
- 文献和实验
- 技术资料
- 库存:
18
- 英文名:
Acylase
- CAS号:
9012-37-7
- 保质期:
2年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
-20℃
- 规格:
25g
- 英文名:
- Acylase
- 别名:
- 酰化酶;L-氨基酰化酶
- CAS号:
- 9012-37-7
- MDL:
- MFCD00081285
- 介绍: Enzyme Commission (EC) Number: 3.5.1.14 ( BRENDA | IUBMB )
- 活力定义:1U:That amount of enzyme which catalyzes the hydrolysis of one micromole of N-Acetyl-L-Methionine per hour at 25° C, pH 7.0.
- 简介:Acylase 1 is an extremely stable enzyme in the dry state. In solution, the enzyme is stable at high temperatures (70° C) at pH 7, but below pH 5 it is rapidly and irreversibly inactivated. The enzyme shows a high degree of optical specificity toward its substrates. For this reason, it has been used extensively for the resolution of racemic amino acids.
- 储存条件: -20℃
- 注意:部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。
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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
isoenzymesS, 60 mg/L, α-MF[83]Kurtzmanomyces sp. 1-11 lipaseS, 46U/ml, α-MF, active464Metarhizium anisopliae chymotrypsinS, α-MF, active322Mucor pusillus renninS, active310Neurospora crassa NOP-1I, 2.2 mg/L, PHO1, active312Neocallimastix patriciarum cellulase
Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
(1992) The oligonucleotides we use are kits, contaning 20 decamers, from Operon Technologies Inc. . I. DNase I treatment of RNA mix and incubate for 30 min. at 37 °C : 50 µgRNA, 10 µl RNase inhibitor (1 U/µl), 1 µl
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