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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
) in balanced saline till fully melted. Set on a hot plate to keep it melted during use for mounting. 2) A drop of molten agarose is placed on a glass slide and flattened with another glass slide into a pad approximately 200 um thick (a single
Allison C. Mallory, Bartel Lab Whitehead InstituteFor 1% Agarose Gel:StockAgarose1g10 X FF buffer10mlwater 88ml1. Boil to dissolve agarose2. Cool it to about 60℃3. Add formaldehyde to a final concentration of 7% (18ml of lab 40% stock) and mix
Preparation of Poly A+ RNA and Northern Analysis
at 56°C and pour denaturing gel (mid-size gel: to 0.8 gm of agarose, add 78.4 ddH2O and 5 ml of 20 x E buffer; heat to dissolve; let cool to not too hot to touch, then quickly add 16.6 ml of 37% formaldehyde, swirl to thoroughly mix and pour). Spin
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