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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ 至 -80℃
- 保质期:
12个月
- 英文名:
Influenza A H1N2 (A/swine/England/038712/2012) Hemagglutinin / HA Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Hemagglutinin/HA蛋白, Hemagglutinin/HA protein
蛋白构建:A DNA sequence encoding the Influenza A virus (A/swine/England/038712/2012 (H1N2)) hemagglutinin (AKJ81614) (Met1-Ser524), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ala 17
蛋白分子量:The recombinant hemagglutinin of the Influenza A virus (A/swine/England/038712/2012 (H1N2)) consists of 519 amino acids and predicts a molecular mass of 59 kDa.
蛋白NP号:AKJ81614
蛋白氨基酸序列:Met1-Ser524
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Two-hybrid analysis of genetic
).The most commonly used prey plasmid,pJG4-5,expresses proteins fused to the B42 activation domain,the SV40 nuclear localization signal,and an epitope tag derived from hemagglutinin,all driven by the yeast GAL1 promoter which is active only in yeast grown on galactose
Two-hybrid analysis of genetic regulatory networks
, and an epitope tag derived from hemagglutinin, all driven by the yeast GAL1 promoter which is active only in yeast grown on galactose (Gyuris et al., 1993). Use of the GAL1 promoter to express the prey allows toxic proteins to be expressed transiently and helps
肉汤培养基,11份PCR检测Mg-DNA阳性的尿道分泌物标本中,有6份在其中呈阳性生长。 国内学者对SP-4培养基进行了改良。赵季文等[4]利用改良的SP-4培基从性病及性乱人群中分离Mg获得成功,初代生长时间在30天以上,平均为37.83天,最长为55天。 1.2组织细胞培养法 组织细胞培养支原体,可为支原体提供良好的类似体内的生长环境。早在60年代,Chanock等[5]报道了肺炎支原体(Mp)在组织细胞中的生长。90年代,Jensen等[3,6]开始尝试用细胞
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