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- 保存条件:
见说明书
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见说明书
- 库存:
200
- 供应商:
齐一生物
- 规格:
1 mg
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文献和实验Alkaline Lysis Plasmid Prep(碱裂解法制备质粒DNA)
the remainder of each culture at 1500xg for 5 min. Pour off the supernatant and resuspend the bacterial cell pellet in 200 µl of GTE buffer (50 mM glucose, 25 mM Tris pH 8, 10 mM EDTA). If you don’t want RNA , add 1μl of 1 mg/ml RNA se to each suspension
of homogenization buffer (4 ml per g) and disperse the tissue in it. 4) Leave for 10 min at room temperature. 5) Add 2 ml of phenol/chloroform and vortex. OR, 1) Put a small or medium sized leaf into a 4" x 6" 500 guage (thick!) plastic bag. 2) Add
Isolate partially purified chromosomal DNA using a modification of the Magic MiniPreps DNA Purification System from Promega (see atta ched) as follows. - Spin down 500 μl of an overnight culture in a 1.5 ml microfuge tube. - Resuspend
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